Selected Publications
Computational protein design and structure prediction – The 2024 Nobel prize in chemistry. Peilong Lu, Lei Liu. Sci. Chem. China, 2024. https://doi.org/10.1007/s11426-024-2469-0
Application of chemical protein synthesis in the study of protein ubiquitination. Shidian Wu, Jiawei Liang, Xiangwei Wu, Zhiheng Deng, Lei Liu*. Sci. Sin. Chim., 2024. https://doi.org/10.1360/SSC-2024-0225
RAD18-catalysed formation of ubiquitination intermediate mimic of proliferating cell nuclear antigen PCNA. Liying Zhang, Zhiheng Deng, Yunxiang Du, Ziyu Xu, Tianyi Zhang, Zebin Tong, Huasong Ai, Lu-Jun Liang*, Lei Liu*. Bioorg Med Chem. 2024 Nov 20;117:118016. https://doi.org/10.1016/j.bmc.2024.118016
Mechanism of nucleosomal H2A K13/15 monoubiquitination and adjacent dual monoubiquitination by RNF168. Huasong Ai, Zebin Tong, Zhiheng Deng, Qiang Shi, Shixian Tao, Gaoge Sun, Jiawei Liang, Maoshen Sun, Xiangwei Wu, Qingyun Zheng, Lujun Liang, Hang Yin, Jia-Bin Li, Shuai Gao, Changlin Tian*, Lei Liu*, Man Pan*. Nat. Chem. Biol., 2024. https://doi.org/10.1038/s41589-024-01750-x
Promotion of RNF168-mediated nucleosomal H2A ubiquitylation by structurally defined K63-polyubiquitylated linker histone H1. Qiang Shi, Zhiheng Deng, Liying Zhang, Zebin Tong, Jia-Bin Li, Guo-Chao Chu*, Huasong Ai*, Lei Liu*. Angew. Chem. Int. Ed., 2024, e202413651. https://doi.org/10.1002/anie.202413651
Ferricyanide-promoted oxidative activation and ligation of protein thioacids in neutral aqueous media. Guo-Chao Chu, Lu-Jun Liang, Rui Zhao, Yan-Yan Guo, Chun-Tong Li, Chong Zuo, Huasong Ai, Xiao Hua, Zi-Chen Li, Yi-Ming Li, Lei Liu*. CCS Chemistry, 2024, 6, 2031–2043. https://doi.org/10.31635/ccschem.023.202303397
Accurate de novo design of heterochiral protein–protein interactions. Ke Sun, Sicong Li, Bowen Zheng, Yanlei Zhu, Tongyue Wang, Mingfu Liang, Yue Yao, Kairan Zhang, Jizhong Zhang, Hongyong Li, Dongyang Han, Jishen Zheng, Brian Coventry, Longxing Cao, David Baker, Lei Liu*, Peilong Lu*. Cell Res., 2024. https://doi.org/10.1038/s41422-024-01014-2
Mirror-image protein and peptide drug discovery through mirror-image phage display. Yun-Kun Qi, Ji-Shen Zheng*, Lei Liu*. Chem, 2024, 10, 2930–2407. https://doi.org/10.1016/j.chempr.2024.06.004
Chemical synthesis of human proteoforms and application in biomedicine. Huasong Ai, Man Pan, Lei Liu*. ACS Cent. Sci., 2024, 10, 1442–1459. https://doi.org/10.1021/acscentsci.4c00642
Enhanced native chemical ligation by peptide conjugation in trifluoroacetic acid. Dong-Liang Huang, Wu-Chen Guo, Wei-Wei Shi, Yun-Pu Gao, Yong-Kang Zhou, Long-Jie Wang, Chen Wang, Shan Tang*, Lei Liu*, Ji-Shen Zheng*. Sci. Adv., 2024, 10, eado9413. https://doi.org/10.1126/sciadv.ado9413
Structural and mechanistic basis for nucleosomal H2AK119 deubiquitination by single-subunit deubiquitinase USP16. Huasong Ai, Zaozhen He, Zhiheng Deng, Guo-Chao Chu, Qiang Shi, Zebin Tong, Jia-Bin Li, Man Pan*, Lei Liu*. Nat. Struct. Mol. Biol., 2024, 31, 1745–1755. https://doi.org/10.1038/s41594-024-01342-2
Recent advances in chemical protein synthesis: method developments and biological applications. Suwei Dong, Ji-Shen Zheng, Yiming Li, Huan Wang, Gong Chen, Yongxiang Chen, Gemin Fang, Jun Guo, Chunmao He, Honggang Hu, Xuechen Li, Yanmei Li, Zigang Li, Man Pan, Shan Tang, Changlin Tian, Ping Wang, Bian Wu, Chuanliu Wu, Junfeng Zhao, Lei Liu*. Sci. China Chem., 2024, 67, 1060–1096. https://doi.org/10.1007/s11426-024-1950-1
Structure-guided engineering enables E3 ligase-free and versatile protein ubiquitination via UBE2E1. Xiangwei Wu, Yunxiang Du, Lu-Jun Liang, Ruichao Ding, Tianyi Zhang, Hongyi Cai, Xiaolin Tian, Man Pan*, Lei Liu*. Nat. Commun., 2024, 15, 1266. https://doi.org/10.1038/s41467-024-45635-y
Synovial sarcoma X breakpoint 1 protein uses a cryptic groove to selectively recognize H2AK119Ub nucleosomes. Zebin Tong, Huasong Ai*, Ziyu Xu, Kezhang He, Guo-Chao Chu, Qiang Shi, Zhiheng Deng, Qiaomei Xue, Maoshen Sun, Yunxiang Du, Lujun Liang, Jia-Bin Li, Man Pan*, Lei Liu*. Nat. Struct. Mol. Biol., 2024, 31, 300–310. https://doi.org/10.1038/s41594-023-01141-1
Cell-permeable stimuli-responsive ubiquitin probe for time-resolved monitoring of substrate ubiquitination in live cells. Lu-Jun Liang, Yu Wang, Xiao Hua, Rujing Yuan, Qiong Xia, Rongtian Wang, Chuntong Li, Guo-Chao Chu, Lei Liu*, Yi-Ming Li*. JACS Au, 2023, 3, 2873–2882. https://doi.org/10.1021/jacsau.3c00421
Mechanistic insights into nucleosomal H2B monoubiquitylation mediated by yeast Bre1-Rad6 and its human homolog RNF20/RNF40-hRAD6A. Zhiheng Deng, Huasong Ai, Maoshen Sun, Zebin Tong, Yunxiang Du, Qian Qu, Liying Zhang, Ziyu Xu, Shixian Tao, Qiang Shi, Jia-Bin Li, Man Pan, Lei Liu*. Mol. Cell, 2023, 83, 3080–3094. https://doi.org/10.1016/j.molcel.2023.08.001
Chemical mechanisms of nucleosomal histone ubiquitination by RING-type E3 enzymes. Qiang Shi, Zebin Tong, Zhiheng Deng, Ziyu Xu, Huasong Ai, Yang Liu, Lei Liu*. Sci. Sin. Chim., 2023, 53, 1455–1471. https://doi.org/10.1360/SSC-2023-0066
Backbone-installed split intein-assisted ligation for the chemical synthesis of mirror-image proteins. Baochang Zhang, Yupeng Zheng, Guochao Chu, Xiangyu Deng, Tongyue Wang, Weiwei Shi, Yongkang Zhou, Shan Tang, Ji-Shen Zheng*, Lei Liu*. Angew. Chem. Int. Ed., 2023, 62, e202306270. https://doi.org/10.1002/anie.202306270
Synthetic E2-Ub-nucleosome conjugates for studying nucleosome ubiquitination. Huasong Ai, Zebin Tong, Zhiheng Deng, Jiakun Tian, Liying Zhang, Maoshen Sun, Yunxiang Du, Ziyu Xu, Qiang Shi, Lujun Liang, Qingyun Zheng, Jia-Bin Li, Man Pan*, Lei Liu*. Chem, 2023, 9, 1221-1240. https://doi.org/10.1016/j.chempr.2023.01.012
Paralog-specific recognition. Huasong Ai, Lei Liu*. Nat. Chem. Biol., 2023, 19, 542-543. https://doi.org/10.1038/s41589-022-01241-x
Simultaneous capture of ISG15 conjugating and deconjugating enzymes using a semi-synthetic ISG15-Dha probe. Chuntong Li, Tian Wang, Lujun Liang, Guochao Chu, Jiachen Zhang, Wei He*, Lei Liu*, Jinghong Li*. Sci. China Chem., 2023, 66, 837-844. https://doi.org/10.1007/s11426-022-1455-x
Single-shot solid-phase synthesis of full-length H2 relaxin disulfide surrogates. Rui Zhao, Pan Shi, Ji-bin Cui, Chaowei Shi, Xiao-Xiong Wei, Jie Luo, Zhemin Xia, Wei-Wei Shi, Yingxin Zhou, Jiahui Tang, Changlin Tian, Mark Meininghaus, Donald Bierer, Jing Shi, Yiming Li, Lei Liu*. Angew. Chem. Int. Ed., 2023, 62, e202216365. https://doi.org/10.1002/anie.202216365
Chemical synthesis of post-translationally modified H2AX reveals redundancy in interplay between histone phosphorylation, ubiquitination, and methylation on the binding of 53BP1 with nucleosomes. Huasong Ai, Guo-Chao Chu, Qingyue Gong, Ze-Bin Tong, Zhiheng Deng, Xin Liu, Fan 5Yang, Ziyu Xu, Jia-Bin Li*, Changlin Tian*, Lei Liu*. J. Am. Chem. Soc., 2022, 144, 18329-18337. https://doi.org/10.1021/jacs.2c06156
Expedient synthesis of ubiquitin-like protein ISG15 tools through chemo-enzymatic ligation catalyzed by a viral protease Lbpro. Tian Wang, Chuntong Li, Meijing Wang, Jiachen Zhang, Qingyun Zheng, Lujun Liang, Guochao Chu, Xiaolin Tian, Haiteng Deng, Wei He*, Lei Liu*, Jinghong Li*. Angew. Chem. Int. Ed., 2022, e202206205. https://doi.org/10.1002/anie.202206205
Recent progress in dissecting ubiquitin signals with chemical biology tools. Qingyun Zheng, Zhen Su, Yuanyuan Yu*, Lei Liu*. Curr. Opin. Chem. Biol., 2022, 70, 102187. https://doi.org/10.1016/j.cbpa.2022.102187
H2B Lys34 ubiquitination induces nucleosome distortion to stimulate Dot1L activity. Huasong Ai, Maoshen Sun, Aijun Liu, Zixian Sun, Tingting Liu, Lin Cao, Lujun Liang, Qian Qu, Zichen Li, Zhiheng Deng, Zebin Tong, Guochao Chu, Xiaolin Tian, Haiteng Deng, Suwen Zhao, Jia-Bin Li*, Zhiyong Lou*, Lei Liu*. Nat. Chem. Biol., 2022, 18, 972-980. https://doi.org/10.1038/s41589-022-01067-7
Structural insights into the activation of somatostatin receptor 2 by cyclic SST analogues. Qing Bo, Fan Yang, Yingge Li, Xianyu Meng, Huanhuan Zhang, Yingxin Zhou, Shenglong Ling, Demeng Sun, Pei Lv, Lei Liu*, Pan Shi* & Changlin Tian*. Cell Discov., 2022, 8, 47. https://doi.org/10.1038/s41421-022-00405-2
Thioester-assisted Sortase-A – mediated ligation. Chong Zuo, Ruichao Ding, Xiangwei Wu, Yuanxia Wang, Guo-Chao Chu, Lu-Jun Liang, Huasong Ai, Ze-Bin Tong, Junxiong Mao, Qingyun Zheng, Tian Wang, Zichen Li, Lei Liu*, Demeng Sun*. Angew. Chem. Int. Ed., 2022, 61, e202201887. https://doi.org/10.1002/anie.202201887
Structural insights into thyrotropin-releasing hormone receptor activation by an endogenous peptide agonist or its orally administered analogue. Fan Yang, Huanhuan Zhang, Xianyu Meng, Yingge Li, Yingxin Zhou, Shenglong Ling, Demeng Sun, Pei Lv, Lei Liu*, Pan Shi*, Changlin Tian*. Cell Research, 2022, 32, 858861. https://doi.org/10.1038/s41422-022-00646-6
Total chemical synthesis of correctly folded disulfide-rich proteins using a removable O-linked β-N-acetylglucosamine strategy. Wei-Wei Shi, Chaowei Shi, Tong-Yue Wang, Yu-Lei Li, Yong-Kang Zhou, Xu-Han Zhang, Donald Bierer, Ji-Shen Zheng*, Lei Liu*. J. Am. Chem. Soc., 2022, 144, 349-357. https://doi.org/10.1021/jacs.1c10091
Structural insights into Ubr1-mediated N-degron polyubiquitination. Man Pan*, Qingyun Zheng, Tian Wang, Lujun Liang, Junxiong Mao, Chong Zuo, Ruichao Ding, Huasong Ai, Yuan Xie, Dong Si, Yuanyuan Yu*, Lei Liu*, Minglei Zhao*. Nature, 2021, 600, 334-338. https://doi.org/10.1038/s41586-021-04097-8
Chemical synthesis of a full-length G‐protein-coupled receptor β2‐adrenergic receptor with defined modification patterns at the C‐terminus. Yulei Li, Jie Heng, Demeng Sun, Baochang Zhang, Xin Zhang, Yupeng Zheng, Wei-Wei Shi, Tong-Yue Wang, Jiu-Yi Li, Xiaoou Sun, Xiangyu Liu, Ji-Shen Zheng*, Brian K. Kobilka, Lei Liu*. J. Am. Chem. Soc., 2021, 143, 17566-17576. https://doi.org/10.1021/jacs.1c07369
Use of a removable backbone modification strategy to prevent aspartimide formation in the synthesis of Asp lactam cyclic peptides. Tingting Cui, Junyou Chen, Rui Zhao, Yanyan Guo, Jiahui Tang, Yulei Li, Yi-Ming Li*, Donald Bierer, Lei Liu*. Chin. J. Chem., 2021, 39, 2517-2522. http://dx.doi.org/10.1002/cjoc.202100272
Mechanistic insight into substrate processing and allosteric inhibition of human p97. Man Pan, Yuanyuan Yu, Huasong Ai, Qingyun Zheng, Yuan Xie, Lei Liu*, Minglei Zhao*. Nat. Struct. Mol. Biol., 2021, 28, 614-625. https://doi.org/10.1038/s41594-021-00617-2
K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle. Yuanyuan Yu, Qingyun Zheng, Satchal K. Erramilli, Man Pan, Seongjin Park, Yuan Xie, Jingxian Li, Jingyi Fei, Anthony A. Kossiakoff*, Lei Liu*, Minglei Zhao*. Nat. Chem. Biol., 2021, 17, 896-905. https://doi.org/10.1038/s41589-021-00823-5
Chemical synthesis of activity-based E2-ubiquitin probes for the structural analysis of E3 ligase-catalyzed transthiolation. Lu-Jun Liang, Guo-Chao Chu, Qian Qu, Chong Zuo, Junxiong Mao, Qingyun Zheng, Jingnan Chen, Xianbin Meng, Yangwode Jing, Haiteng Deng, Yi-Ming Li, Lei Liu*. Angew. Chem. Int. Ed., 2021, 60, 17171-17177. https://doi.org/10.1002/anie.202105870
A mirror-image protein-based information barcoding and storage technology. Ji-Shen Zheng, Jun Liang, Wei-Wei Shi, Ying Li, Hong-Gang Hu, Chang-Lin Tian, Lei Liu*. Science Bulletin, 2021, 66, 1542-1549. https://doi.org/10.1016/j.scib.2021.03.010
Structural basis of human α7 nicotinic acetylcholine receptor activation. Yue Zhao, Sanling Liu*, Yingxin Zhou, Mengge Zhang, Haopeng Chen, H. Eric Xu, Demeng Sun*, Lei Liu*, Changlin Tian*. Cell Research, 2021, 31, 713-716. https://doi.org/10.1038/s41422-021-00509-6
Structural mechanism of cooperative activation of the human calcium-sensing receptor by Ca2+ ions and L-tryptophan. Shenglong Ling, Pan Shi, Sanling Liu, Xianyu Meng, Yingxin Zhou, Wenjing Sun, Shenghai Chang, Xing Zhang, Longhua Zhang, Chaowei Shi, Demeng Sun*, Lei Liu*, Changlin Tian*. Cell Research, 2021, 31, 383-394. https://doi.org/10.1038/s41422-021-00474-0
Seesaw conformations of Npl4 in the human p97 complex and the inhibitory mechanism of a disulfiram derivative. Man Pan, Qingyun Zheng, Yuanyuan Yu, Huasong Ai, Yuan Xie, Xin Zeng, Chu Wang, Lei Liu*, Minglei Zhao*. Nat. Commun., 2021, 12, 121. https://doi.org/10.1038/s41467-020-20359-x
Chemical synthesis of proteins containing 300 amino acids. Baochang Zhang, Yulei Li, Weiwei Shi, Tongyue Wang, Feng Zhang, Lei Liu*. Chemical Research in Chinese Universities, 2020, 36, 733-747. https://doi.org/10.1007/s40242-020-0150-y
Structural insights into human acid-sensing ion channel 1a inhibition by snake toxin mambalgin1. Demeng Sun, Sanling Liu, Siyu Li, Mengge Zhang, Fan Yang, Ming Wen, Pan Shi, Tao Wang, Man Pan, Shenghai Chang, Xing Zhang, Longhua Zhang*, Changlin Tian*, Lei Liu*. eLife, 2020, 9: e57096. https://doi.org/10.7554/eLife.57096
Development and application of ubiquitin-based chemical probes. Xin Sui, Yu Wang, Yun-Xiang Du, Lu-Jun Liang, Qingyun Zheng, Yi-Ming Li*, Lei Liu*. Chem. Sci., 2020, 11, 12633-12646. https://doi.org/10.1039/D0SC03295F
A diaminodiacid (DADA) strategy for the development of disulfide surrogate peptides. Yun-Kun Qi, Qian Qu, Donald Bierer, Lei Liu*. Chem. Asian J., 2020, 15, 2793-2802. https://doi.org/10.1002/asia.202000609
An E1-catalyzed chemoenzymatic strategy to isopeptide-N-ethylated deubiquitylase-resistant ubiquitin probes. Qingyun Zheng, Tian Wang, Guo-Chao Chu, Chong Zuo, Rui Zhao, Xin Sui, Linzhi Ye, Yuanyuan Yu, Jingnan Chen, Xiangwei Wu, Wenhao Zhang, Haiteng Deng, Jing Shi, Man Pan*, Yi-Ming Li*, Lei Liu*. Angew. Chem. Int. Ed., 2020, 59, 13496-13501. https://doi.org/10.1002/anie.202002974
A novel D-peptide identified by mirror-image phage display blocks TIGIT/PVR for cancer immunotherapy. Xiuman Zhou, Chao Zuo, Wanqiong Li, Weiwei Shi, Xiaowen Zhou, Hongfei Wang, Shaomeng Chen, Jiangfeng Du, Guanyu Chen, Wenjie Zhai, Wenshan Zhao, Yahong Wu, Yuanming Qi, Lei Liu*, Yanfeng Gao*. Angew. Chem. Int. Ed., 2020, 59, 15114-15118. https://doi.org/10.1002/anie.202002783
Advances on structural mechanisms of ubiquitinated nucleosome complexes. Ziyu Xu, Huasong Ai, Maoshen Sun, Jiakun Tian, Lei Liu*. Sci. Sin. Chim., 2020, 50, 415-436. http://engine.scichina.com/publisher/scp/journal/SSC/50/4/10.1360/SSC-2019-0170?slug=fulltext
Synthesis of disulfide surrogate peptides incorporating large-span surrogate bridges through a native-chemical-ligation-assisted diaminodiacid strategy. Qian Qu, Shuai Gao, Fangming Wu, Meng-Ge Zhang, Ying Li, Long-Hua Zhang, Donald Bierer, Chang-Lin Tian*, Ji-Shen Zheng*, Lei Liu*. Angew. Chem. Int. Ed., 2020, 59, 6037-6045. https://doi.org/10.1002/anie.201915358
Identification of interferon receptor IFNAR2 as a novel HCV entry factor by using chemical probes. Jie Qing, Ming Wu, Rui Luo, Jizheng Chen, Lin Cao, Debin Zeng, Luqing Shang, Junxiu Nong, Qinkai Wu, Bi-Sen Ding, Xinwen Chen*, Zihe Rao*, Lei Liu*, Zhiyong Lou*. ACS Chem. Biol., 2020, 15, 1232-1241. https://doi.org/10.1021/acschembio.9b00912
Inactivity of YGL082W in vitro due to impairment of conformational change in the catalytic center loop. Lining Lu, Yu Guo, Tian Wang, Lujun Liang, Suwen Zhao*, Feng Wang*, Lei Liu*. Sci. China Chem., 2020, 63, 237-243. https://link.springer.com/article/10.1007%2Fs11426-019-9623-0
Chemical protein synthesis enabled mechanistic studies on the molecular recognition of K27-linked ubiquitin chains. Man Pan, Qingyun Zheng, Shan Ding, Lujia Zhang, Qian Qu, Tian Wang, Danning Hong, Yujing Ren, Lujun Liang, Chunlai Chen*, Ziqing Mei*, Lei Liu*. Angew. Chem. Int. Ed., 2019, 58, 2627-2631. https://doi.org/10.1002/anie.201810814
Cysteine-aminoethylation-assisted chemical ubiquitination of recombinant histones. Guo-Chao Chu, Man Pan, Jiabin Li, Sanling Liu, Chong Zuo, Ze-Bin Tong, Jing-Si Bai, Qingyue Gong, Huasong Ai, Jian Fan, Xianbin Meng, Yi-Chao Huang, Jing Shi, Haiteng Deng, Changlin Tian*, Yi-Ming Li*, Lei Liu*. J. Am. Chem. Soc., 2019, 141, 3654-3663. https://doi.org/10.1021/jacs.8b13213
Examination of the deubiquitylation site selectivity of USP51 by using chemically synthesized ubiquitylated histones. Huasong Ai, Yu Guo, Demeng Sun, Sanling Liu, Yunkun Qi, Jing Guo, Qian Qu, Qingyue Gong, Suwen Zhao*, Jiabin Li*, Lei Liu*. ChemBioChem, 2019, 20, 221-229. https://doi.org/10.1002/cbic.201800432
Chemical synthesis of structurally defined phosphorylated ubiquitins suggests impaired Parkin activation by phosphorylated ubiquitins with a non-phosphorylated distal unit. Man Pan, Qingyun Zheng, Shuai Gao, Qian Qu, Yuanyuan Yu, Ming Wu, Huan Lan, Yulei Li, Sanling Liu, Jiabin Li, Demeng Sun, Lining Lu, Tian Wang, Wenhao Zhang, Jiawei Wang, Yiming Li, Hong-Gang Hu*, Changlin Tian*, Lei Liu*. CCS Chem., 2019, 1, 476-489. https://doi.org/10.31635/ccschem.019.20190001
Chimeric protein probes for C5a receptors through fusion of the anaphylatoxin C5a core region with a small-molecule antagonist. Chao Zuo, Wei-Wei Shi, Xiao-Xu Chen, Marie Glatz, Bernd Riedl, Ingo Flamme, Elisabeth Pook, Jiawei Wang, Ge-Min Fang, Donald Bierer, Lei Liu*. Sci. China Chem., 2019, 62, 1371-1378. https://link.springer.com/article/10.1007/s11426-019-9513-2
Ligation of soluble but unreactive peptide segments in the chemical synthesis of Haemophilus influenzae DNA ligase. Baochang Zhang, Qiang Deng, Chong Zuo, Bingjia Yan, Chao Zuo, Xiu-Xiu Cao, Ting F. Zhu, Ji-Shen Zheng*, Lei Liu*. Angew. Chem. Int. Ed., 2019, 58, 12231-12237. https://doi.org/10.1002/anie.201905149
Chemical synthesis of proteins that cannot be obtained recombinantly. Lei Liu*. Isr. J. Chem., 2019, 59, 64-70. https://doi.org/10.1002/ijch.201800135
Selective modification of natural nucleophilic residues in peptides and proteins using arylpalladium complexes. Wan-Min Cheng, Xi Lu, Jing Shi*, Lei Liu*. Org. Chem. Front., 2018, 5, 3186-3193. https://doi.org/10.1039/C8QO00765A
Acyl donors for native chemical ligation. Bingjia Yan, Weiwei Shi, Linzhi Ye, Lei Liu*. Curr. Opin. Chem. Biol., 2018, 46, 33-40. https://doi.org/10.1016/j.cbpa.2018.03.018
Studies on mirror-image proteins. Zichen Li, Baochang Zhang, Chong Zuo, Lei Liu*. Chin. J. Org. Chem., 2018, 38, 2412-2419. http://sioc-journal.cn/Jwk_yjhx/CN/10.6023/cjoc201804014
Semi-synthesis of disulfide-linked branched tri-ubiquitin mimics. Yanyan Si, Lujun Liang, Shan Tang, Yunkun Qi, Yong Huang, Lei Liu*. Sci. China Chem., 2018, 61, 412-417. https://link.springer.com/article/10.1007%2Fs11426-017-9189-6
Cryo-EM structure of the ASIC1a-mambalgin-1 complex reveals that the peptide toxin mambalgin-1 inhibits acid-sensing ion channels through an unusual allosteric effect. Demeng Sun, You Yu, Xiaobin Xue, Man Pan, Ming Wen, Siyu Li, Qian Qu, Xiaorun Li, Longhua Zhang, Xueming Li, Lei Liu*, Maojun Yang*, Changlin Tian*. Cell Discov., 2018, 4, 27. https://doi.org/10.1038/s41421-018-0026-1
Chemical synthesis of membrane proteins by the removable backbone modification method. Shan Tang, Chao Zuo, Dong-Liang Huang, Xiao-Ying Cai, Long-Hua Zhang, Chang-Lin Tian*, Ji-Shen Zheng*, Lei Liu*. Nat. Protoc., 2017, 12, 2554-2569. https://doi.org/10.1038/nprot.2017.129
Mirror-image polymerase chain reaction. Wenjun Jiang, Baochang Zhang, Chuyao Fan, Min Wang, Jiaxing Wang, Qiang Deng, Xianyu Liu, Ji Chen, Jishen Zheng, Lei Liu*, Ting F Zhu*. Cell Discov., 2017, 3, 17037. https://doi.org/10.1038/celldisc.2017.37
Recent advances in racemic protein crystallography. Bingjia Yan, Linzhi Ye, Weiliang Xu, Lei Liu*. Bioorg. Med. Chem., 2017, 25, 4953-4965. https://doi.org/10.1016/j.bmc.2017.05.020
Total chemical synthesis of a thermostable enzyme capable of polymerase chain reaction. Weiliang Xu, Wenjun Jiang, Jiaxing Wang, Linping Yu, Ji Chen, Xianyu Liu, Lei Liu*, Ting F Zhu*. Cell Discov., 2017, 3, 17008. https://doi.org/10.1038/celldisc.2017.8
Practical chemical synthesis of atypical ubiquitin chains by using an isopeptide-linked Ub isomer. Shan Tang, Lu-Jun Liang, Yan-Yan Si, Shuai Gao, Jia-Xing Wang, Jun Liang, Ziqing Mei*, Ji-Shen Zheng*, Lei Liu*. Angew. Chem. Int. Ed., 2017, 56, 13333-13337. https://doi.org/10.1002/anie.201708067
Removable backbone modification method for the chemical synthesis of membrane proteins. Jia-Bin Li, Shan Tang, Ji-Shen Zheng*, Chang-Lin Tian*, Lei Liu*. Acc. Chem. Res., 2017, 50, 1143-1153. https://doi.org/10.1021/acs.accounts.7b00001
A semisynthetic Atg3 reveals that acetylation promotes Atg3 membrane binding and Atg8 lipidation. Yi-Tong Li, Cong Yi, Chen-Chen Chen, Huan Lan, Man Pan, Shao-Jin Zhang, Yi-Chao Huang, Chao-Jian Guan, Yi-Ming Li*, Li Yu*, Lei Liu*. Nat. Commun., 2017, 8, 14846. https://doi.org/10.1038/ncomms14846
Chemical synthesis of histone H2A with methylation at Gln104. Qiaoqiao He, Jiabin Li*, Yunkun Qi, Zhipeng Wang, Yong Huang, Lei Liu*. Sci. China Chem., 2017, 60, 621-627. https://doi.org/10.1007/s11426-016-0386-4
Monomer/oligomer quasi-racemic protein crystallography. Shuai Gao, Man Pan, Yong Zheng, Yichao Huang, Qingyun Zheng, Demeng Sun, Lining Lu, Xiaodan Tan, Xianglong Tan, Huan Lan, Jiaxing Wang, Tian Wang, Jiawei Wang*, Lei Liu*. J. Am. Chem. Soc., 2016, 138, 14497-14502. https://pubs.acs.org/doi/10.1021/jacs.6b09545
Chemical synthesis of proteins using hydrazide intermediates. Yi-Chao Huang, Ge-Min Fang, Lei Liu*. Natl. Sci. Rev., 2016, 3, 107-116. https://doi.org/10.1093/nsr/nwv072
Protein modification: Standing out from the crowd. Yi-Chao Huang, Lei Liu*. Nat. Chem., 2016, 8, 100-101. https://doi.org/10.1038/nchem.2443
A synthetic molecular system capable of mirror-image genetic replication and transcription. Zimou Wang, Weiliang Xu, Lei Liu*, Ting F. Zhu*. Nat. Chem., 2016, 8, 698-704. https://doi.org/10.1038/nchem.2517
Quasi-racemic X-ray structures of K27-linked ubiquitin chains prepared by total chemical synthesis. Man Pan, Shuai Gao, Yong Zheng, Xiaodan Tan, Huan Lan, Xianglong Tan, Demeng Sun, Lining Lu, Tian Wang, Qingyun Zheng, Yichao Huang, Jiawei Wang, Lei Liu*. J. Am. Chem. Soc., 2016, 138, 7429-7435. https://doi.org/10.1021/jacs.6b04031
Total synthesis of mambalgin-1/2/3 by two-segment hydrazide-based native chemical ligation. Huan Lan, Kun Wu, Yong Zheng, Man Pan, Yi-Chao Huang, Shuai Gao, Qing-Yun Zheng, Ji-Shen Zheng*, Yi-Ming Li, Bailong Xiao, Lei Liu*. J. Pept. Sci., 2016, 22, 320-326. https://doi.org/10.1002/psc.2868
Robust chemical synthesis of membrane proteins through a general method of removable Backbone Modification. Ji-Shen Zheng, Yao He, Chao Zuo, Xiao-Ying Cai, Shan Tang, Zhipeng A. Wang, Long-Hua Zhang, Chang-Lin Tian*, Lei Liu*. J. Am. Chem. Soc., 2016, 138, 3553-3561. https://doi.org/10.1021/jacs.6b00515
Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen-antibody interactions. Shan Tang, Zhengpeng Wan, Yiren Gao, Jishen Zheng, Jing Wang, Yanyan Si, Xin Chen, Hai Qi, Lei Liu*, Wanli Liu*. Chem. Sci., 2016, 7, 1891-1895. https://doi.org/10.1039/C5SC03404C
Utilization of a photoactivatable antigen system to examine B-cell probing termination and the B-cell receptor sorting mechanisms during B-cell activation. Jing Wang, Shan Tang, Zhengpeng Wan, Yiren Gao, Yiyun Cao, Junyang Yi, Yanyan Si, Haowen Zhang, Lei Liu*, Wanli Liu*. Proc. Natl. Acad. Sci. USA, 2016, 113, 558-567. https://doi.org/10.1073/pnas.1517612113
KAHA ligation at serine. Yi-Ming Li*, Yi-Chao Huang, Lei Liu*. ChemBioChem, 2016, 17, 28-30. https://doi.org/10.1002/cbic.201500527
Hmboff/on as a switchable thiol protecting group for native chemical ligation. Yun-Kun Qi, Shan Tang, Yi-Chao Huang, Man Pan, Ji-Shen Zheng*, Lei Liu*. Org. Biomol. Chem., 2016, 14, 4194-4198. https://doi.org/10.1039/C6OB00450D
Identification, synthesis and pharmacological evaluation of novel anti-EV71 agents via cyclophilin A inhibition. Wenzhong Yan, Jie Qing, Hanbing Mei, Junxiu Nong, Jin Huang, Jin Zhu, Hualiang Jiang, Lei Liu*, Linqi Zhang*, Jian Li*. Bioorg. Med. Chem. Lett., 2015, 25, 5682-5686. https://doi.org/10.1016/j.bmcl.2015.11.002
Chemical synthesis of crystalline proteins. Yichao Huang, Lei Liu*. Sci. China Chem., 2015, 58, 1779-1781. https://doi.org/10.1007/s11426-015-5462-2
Blocking of the PD-1/PD-L1 Interaction by a D–pepetide Antagonist for Cancer Immunotherapy. Hao-Nan Chang, Bei-Yuan Liu, Yun-Kun Qi, Yang Zhou, Yan-Ping Chen, Kai-Mai Pan, Wen-Wen Li, Xiu-Man Zhou, Wei-Wei Ma, Cai-Yun Fu, Yuan-Ming Qi, Lei Liu*, Yan-Feng Gao*. Angew. Chem. Int. Ed., 2015, 54, 11760-11764. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201506225
Chemical synthesis of a two-photon-activatable chemokine and photon-guided lymphocyte migration in vivo. Xin Chen, Shan Tang, Ji-Shen Zheng, Ruozhu Zhao, Zhi-Peng Wang, Wen Shao, Hao-Nan Chang, Jing-Yuan Cheng, Hui Zhao, Lei Liu*, Hai Qi*. Nat. Commun., 2015, 6, 7220-7228. https://www.nature.com/articles/ncomms8220
An efficient one-pot four-segment condensation method for protein chemical synthesis. Shan Tang, Yan-Yan Si, Zhi-Peng Wang, Kun-Rong Mei, Xin Chen, Jing-Yuan Cheng, Ji-Shen Zheng, Lei Liu*. Angew. Chem. Int. Ed., 2015, 54, 5713-5717. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201500051
Peptide o-aminoanilides as crypto-thioesters for protein chemical synthesis. Jia-Xing Wang, Ge-Min Fang, Yao He, Da-Liang Qu, Min Yu, Zhang-Yong Hong, Lei Liu*. Angew. Chem. Int. Ed., 2015, 54, 2194-2198. https://www.onlinelibrary.wiley.com/doi/abs/10.1002/anie.201408078
Diaminodiacid bridges to improve folding and tune the bioactivity of disulfide-rich peptides. Ye Guo, De-Meng Sun, Feng-Liang Wang, Yao He, Lei Liu*, Chang-Lin Tian*. Angew. Chem. Int. Ed., 2015, 54, 14276-14281. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201500699
Discovery of novel small molecule anti-HCV agents via the CypA inhibitory mechanism using O-acylation-directed lead optimization. Wenzhong Yan, Jie Qing, Hanbing Mei, Fei Mao, Jin Huang, Jin Zhu, Hualiang Jiang, Lei Liu*, Linqi Zhang*, Jian Li*. Molecules, 2015, 20, 10342-10359. https://www.mdpi.com/1420-3049/20/6/10342
Irreversible site-specific hydrazinolysis of proteins by use of sortase. Yi-Ming Li, Yi-Tong Li, Man Pan, Xiu-Qi Kong, Yi-Chao Huang, Zhang-Yong Hong, Lei Liu*. Angew. Chem. Int. Ed., 2014, 53, 2198-2202. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201310010
Synthesis of and specific antibody generation for glycopeptides with Arginine N-GlcNacylation. Man Pan, Shan Li, Xiang Li, Feng Shao*, Lei Liu*, Hong-Gang Hu*. Angew. Chem. Int. Ed., 2014, 53, 14517-14521. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201407824
Cyclophilin A associates with enterovirus-71 virus capsid and plays an essential role in viralinfection as an uncoating regulator. Jie Qing, Yaxin Wang, Yuna Sun, Jiaoyan Huang, Wenzhong Yan, Jinglan Wang, Dan Su, Cheng Ni, Jian Li, Zihe Rao, Lei Liu*, Zhiyong Lou*. PLoS Pathog., 2014, 10, e1004422. https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1004422
One-pot native chemical ligation of peptide hydrazides enables total synthesis of modified histones. Jiabin Li, Yuanyuan Li, Qiaoqiao He, Yiming Li*, Haitao Li, Lei Liu*. Org. Biomol. Chem., 2014, 12, 5435-5441. https://pubs.rsc.org/en/content/articlelanding/2014/ob/c4ob00715h#!divAbstract
Expedient total synthesis of small to medium-sized membrane proteins via Fmoc chemistry. Ji-Shen Zheng, Mu Yu, Yun-Kun Qi, Shan Tang, Fei Shen, Zhi-Peng Wang, Liang Xiao, Longhua Zhang, Chang-Lin Tian*, Lei Liu*. J. Am. Chem. Soc., 2014, 136, 3695–3704. https://pubs.acs.org/doi/abs/10.1021/ja500222u
New semi-synthesis of ubiquitin C-terminal conjugate with 7-amino-4-methylcoumarin. Yi-Tong Li, Jun Liang, Jia-Bin Li, Ge-Min Fang, Yong Huang, Lei Liu*. J. Pept. Sci., 2014, 20, 102-107. https://onlinelibrary.wiley.com/doi/abs/10.1002/psc.2568
Facile solid-phase synthesis of PNA-peptide conjugates using pNZ-protected PNA monomers. Yi-Chao Huang, Cheng Cao, Xiang-Long Tan, Xiaoyu Li*, Lei Liu*. Org. Chem. Front., 2014, 1, 1050-1054. https://doi.org/10.1039/C4QO00217B
Chemical synthesis of proteins using peptide hydrazides as thioester surrogates. Ji-Shen Zheng, Shan Tang, Yun-Kun Qi, Zhi-Peng Wang, Lei Liu*. Nat. Protoc., 2013, 8, 2483-2495. https://www.nature.com/articles/nprot.2013.152
Diaminodiacid-based solid-phase synthesis of peptide disulfide bond mimics. Hong-Kui Cui, Ye Guo, Yao He, Feng-Liang Wang, Hao-Nan Chang, Yu-Jia Wang, Fang-Ming Wu, Chang-Lin Tian*, Lei Liu*. Angew. Chem. Int. Ed., 2013, 52, 9558-9562. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201302197
Chemical synthesis of Ub-AMC via ligation of peptide hydrazides. Jun Liang, GeMin Fang, XiuLiang Huang, ZiQing Mei, Juan Li, ChangLin Tian*, Lei Liu*. Sci. China Chem., 2013, 56, 1301-1306. https://link.springer.com/article/10.1007/s11426-013-4885-x
Development of new thioester equivalents for protein chemical synthesis. Ji-Shen Zheng, Shan Tang, Yi-Chao Huang, Lei Liu*. Acc. Chem. Res., 2013, 46, 2475-2484. https://pubs.acs.org/doi/10.1021/ar400012w
Synthesis of autophagosomal marker protein LC3-II under detergent-free conditions. Yi-Chao Huang, Yi-Ming Li, Yang Chen, Man Pan, Yi-Tong Li, Li Yu, Qing-Xiang Guo, Lei Liu*. Angew. Chem. Int. Ed., 2013, 52, 4858-4862. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201209523
Design of thiol-containing amino acids for native chemical ligation at non-Cys sites. Qiao-Qiao He, Ge-Min Fang, Lei Liu*. Chin. Chem. Lett., 2013, 24, 265-269. https://www.sciencedirect.com/science/article/abs/pii/S1001841713001356
Design of stapled α-helical peptides to specifically activate Wnt/β-catenin signaling. Hong-Kui Cui, Bing Zhao, Yehua Li, Ye Guo, Hao Hu, Lei Liu*, Ye-Guang Chen*. Cell Res., 2013, 23, 581-584. https://www.nature.com/articles/cr201330
Stapled peptide-based membrane fusion inhibitors of hepatitis C virus. Hong-Kui Cui, Jie Qing, Ye Guo, Yu-Jia Wang, Li-Jia Cui, Tian-Hua He, Linqi Zhang*, Lei Liu*. Bioorg. Med. Chem. Lett., 2013, 21, 3547-3554. https://www.sciencedirect.com/science/article/pii/S0968089613001351
Convergent chemical synthesis of proteins by ligation of peptide hydrazides. Ge-Min Fang, Jia-Xing Wang, Lei Liu*. Angew. Chem. Int. Ed., 2012, 51, 10347-10350. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201203843
Genetically encoded alkenyl-pyrrolysine analogues for thiol–ene reaction mediated site-specific protein labelling. Yiming Li, Maiyun Yang, Yichao Huang, Xiaoda Song, Lei Liu*, Peng R. Chen*. Chem. Sci., 2012, 9, 2766-2770. https://pubs.rsc.org/en/content/articlelanding/2012/sc/c2sc20433a#!divAbstract
Ligation of expressed protein α-hydrazides via genetic incorporation of an α-hydroxy acid. Yi-Ming Li, Mai-Yun Yang, Yi-Chao Huang, Yi-Tong Li, Peng R. Chen*, Lei Liu*. ACS Chem. Biol., 2012, 7, 1015-1022. https://pubs.acs.org/doi/10.1021/cb300020s
Synthesis of cyclic peptides and cyclic proteins via ligation of peptide hydrazides. Ji-Shen Zheng, Shan Tang, Ye Guo, Hao-Nan Chang, Lei Liu*. ChemBioChem, 2012, 13, 542-546. https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/cbic.201100580
Mechanism of imidazole-promoted ligation of peptide phenyl esters. Chen Wang, Lei Liu*. Chin. J. Chem., 2012, 30, 1974-1979. https://onlinelibrary.wiley.com/doi/10.1002/cjoc.201200337
Synthesis of cyclic tetrapeptides via ligation of peptide hydrazides. Shan Tang, Jishen Zheng, Ke Yang, Lei Liu*. Acta Chim. Sin., 2012, 70, 1471-1476. http://sioc-journal.cn/Jwk_hxxb/CN/10.6023/A12040166
Chemical synthesis of a cyclotide via intramolecular cyclization of peptide O-esters. Ji-Shen Zheng, Hao-Nan Chang, Jing Shi*, Lei Liu*. Sci. China Chem., 2012, 55, 64-69. https://link.springer.com/article/10.1007%2Fs11426-011-4434-4
Chemical synthesis of integral membrane proteins: Methods and applications. Fei Shen, Yi-Chao Huang, Shan Tang, Yong-Xiang Chen, Lei Liu*. Isr. J. Chem., 2011, 51, 940-952. https://onlinelibrary.wiley.com/doi/abs/10.1002/ijch.201100076
Fmoc synthesis of peptide thioesters without post-chain-assembly manipulation. Ji-Shen Zheng, Hao-Nan Chang, Feng-Liang Wang, Lei Liu*. J. Am. Chem. Soc., 2011, 133, 11080-11083. https://pubs.acs.org/doi/abs/10.1021/ja204088a
Hydrazine-sensitive thiol protecting group for peptide and protein chemistry. Fei Shen, Zhi-Ping Zhang, Jia-Bin Li, Yun Lin, Lei Liu*. Org. Lett., 2011, 13, 568-571. https://pubs.acs.org/doi/10.1021/ol1028755
Hexafluoro-2-propanol as a potent cosolvent for chemical ligation of membrane proteins. Fei Shen, Shan Tang, Lei Liu*. Sci. China Chem., 2011, 54, 110-116. https://link.springer.com/article/10.1007%2Fs11426-010-4188-4
Protein chemical synthesis by ligation of peptide hydrazides. Ge-Min Fang, Yi-Ming Li, Fei Shen, Yi-Chao Huang, Jia-Bin Li, Yun Lin, Hong-Kui Cui, Lei Liu*. Angew. Chem. Int. Ed., 2011, 50, 7645-7649. https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201100996